Apoptosis in Human Leukemic Cells Induced by Lactoferricin, a Bovine Milk Protein-Derived Peptide: Involvement of Reactive Oxygen Species

摘要: We examined the activity of bovine lactoferricin (Lfcin-B), a peptide derived from milk protein lactoferrin (LF-B), to induce apoptosis in THP-1 human monocytic leukemic cells. Treatment with Lfcin-B at up 50 μg/ml induced cell death cells dose- and time-dependent manner, showing apparent morphological changes, hypodiploid forms genomic DNA apoptotic fragmentation, whereas LF-B was inactive even high dose (500 μg/ml). The apoptosis-inducing effect increased reduction serum concentration, but inhibited by addition Zn2+, inhibitor Ca2+/Mg2+-dependent endonucleases dose-dependent manner. Furthermore, Lfcin-B-induced completely abolished antioxidants such asN-acetyl-l-cysteine (NAC) glutathione (GSH), not various cytokines mitogen which can activate In addition, treated Lfcin-B, LF-B, showed levels intracellular reactive oxygen species (ROS) early period (20 min) treatment. And production ROS dependent upon added. These results suggested that LF-B-derived peptide, itself, is able tumor cells, its related pathway mediated activation endonucleases.