Embryo transfer using cryopreserved Boer goat blastocysts

摘要: The aim of this trial was to evaluate the effect embryo cryopreservation techniques on survivability embryos and fertility following transfer Boer goat does. oestrous cycles 27 mature recipients does were synchronised using controlled internal drug release dispensers (CIDR’s) for 16 days. At CIDR removal, injected with 300 IU eCG. recipient allocated 3 groups (n = 9 per group), based technique used transferred. in vivo produced at blastocyst stage surgically collected day 6 AI from donors superovulated pFSH. first group received fresh served as control, second conventional slow frozen/thawed third vitrified/thawed embryos. Two blastocysts transferred doe. A pregnancy rate 85.7% 6) obtained tended be better than receiving frozen vitrified embryos, 4; 50.0% n 3; 37.5% pregnant, respectively). overall gestation period recorded all 146.3 ± 3.0 d, an litter size 1.7 0.5 being recorded. kidding declined 57.0% (4) 25.0% (2) groups, respectively. An survival 35.7% 5) fresh, 4) freezing 31.3% vitrification not affected by number CL’s present respective ovaries time transfer. There a tendency more females born males (ratio 1 : 2, male female) but could related technique. Although satisfactory, either or cryopreserved less acceptable. More research is warranted larger numbers animals, directed improving