A simple paper test for isoniazid in urine

摘要: Tests for the presence of chemotherapeutic drugs or their metabolites in urine play an important part management the treatment tuberculosis (Dixon et al., 1957; Fox, 1958). A previous report from this Centre (Gangadharam 1958) presented a comparison a number methods detecting isoniazid including direct naphthoquinone-mercuric chloride (N-M) test (Short and Case, 1957), also modification of this test which employed alkaline hydrolysis to liberate isoniazid its conjugated forms. The direct-and hydrolysis N-M tests have been employed Centre past four years to control self-administration isoniazid used domiciliary treatment pulmonary tuberculosis. The effect irregularity in taking as detected by these on the response to has been reported elsewhere (Tuberculosis Chemotherapy Centre, 1960). Since method disadvantage that it requires certain amount equipment and trained personnel, is not suitable for routine use all chest clinics under field conditions. An attempt was therefore made simplify direct by incorporating reagents absorbent papers; Though impregnation paper with the pHl0 buffer naphthoquinone reagent was successful, aqueous solution mercuric unsatisfactory. In 1960, Cattaneo, Fantoli Belasio published details difficulty was overcome impregnating papers with solution ether. Since then modification adopted for preparation test-paper developed in Centre. Since lower concentration naphthoquinone reagent shorter period exposure was used testpaper developed than described Cattaneo al. (1960), both tests have compared combined N-M previously (Gangadharam et This presents the results comparison an of specificity test.