Determination of cichoric acid content in dried press juice of purple coneflower (Echinacea purpurea) with capillary electrophoresis

作者: B MANCEK , S KREFT

DOI: 10.1016/J.TALANTA.2005.01.028

关键词: Cichoric acidPolysaccharideChemistryCapillary electrophoresisCaffeic acidElectrophoresisChromatographyEchinacea

摘要: Abstract Purple coneflower (Echinacea purpurea) is an immunostimulating drug, containing multiple substances. The most important in activity are polysaccharides, caffeic acid derivatives (cichoric acid), alkamides and glycoproteins. It not clear yet, which substances responsible for activity. Cichoric appropriate marker of the quality E. purpurea product, because it has immune stimulatory effects susceptible to degradation. In this work, improved capillary electrophoresis method determining cichoric dried press juice from purple was developed. optimal conditions were: electrophoretic buffer—75 mM borate, pH 8.8; injection 20 mbar 20 s; separation at 20 kV; detection 350 nm, temperature 35 °C.

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