N-Acetyl Aspartic Acid (NAA) and N-Acetyl Aspartylglutamic Acid (NAAG) in Human Ventricular, Subarachnoid, and Lumbar Cerebrospinal Fluid

摘要: N-Acetylaspartic and N-acetylaspartylglutamic acid concentrations in human ventricular, subarachnoid lumbar cerebrospinal fluid were measured by combined gas chromatography-mass spectrometry using selected ion monitoring with deuterated internal standards. N-Acetylaspartate the range 55, 9, 1 μM, respectively; N-acetylaspartylglutamate same fluids 8, 3 4 respectively. There did not appear to be any difference of either compound between control subjects, schizophrenic patients, Alzheimer's disease patients a pooled group neurological degeneration. Ventricular both compounds greatly increased deceased suggesting that maintenance their intracellular is probably energy dependent. The these from living, ventricular subjects weakly correlated one another. In neither appeared age. Analysis serially collected samples two showed weak concentration gradient for compounds. Neither antipsychotic medication nor transport inhibitor probenecid had effect on compound. Attempts use anion exchange high pressure liquid chromatography UV detection measurement low N-acetylaspartate found living unsuccessful.