Comparison of helper and cytotoxic antirecipient T cell frequencies in unrelated bone marrow transplantation.

摘要: Donor/recipient histocompatibility antigen differences initiate acute graft-versus-host disease (GVHD) after bone marrow transplantation. Frequency analysis, using limiting dilution techniques, of functionally defined (helper or cytotoxic) antirecipient T lymphocyte precursors in the peripheral blood donor has been shown to be an accurate predictor for development moderate-to-severe GVHD. Here, we describe a sensitive assay measuring alloreactive helper (IL-2-producing) precursor (HTLp) frequencies, and compare ability this cytotoxic (CTLp) detect HLA- class II I predict clinical outcome cohort unrelated donor/recipient BMT pairs. Twenty-two pairs underwent BMT. Patients with high (> 1:100 x 10(3)) HTLp CTLp frequencies had higher incidence (grades II-IV) GVHD (80% 100%, respectively) than low (< (40% 57%, respectively). Ten (45%) patients have died, but all both frequency remain alive. The assays provided similar predictive information outcome. Given that is more rapid less labor intensive, it offers additional alternative functional method selection