Effects of salinity acclimation on the expression and activity of Phase I enzymes (CYP450 and FMOs) in coho salmon (Oncorhynchus kisutch)

摘要: Phase I biotransformation enzymes are critically important in the disposition of xenobiotics within biota and regulated by multiple environmental cues, particularly anadromous fish species. Given importance these enzyme systems xenobiotic/endogenous chemical bioactivation detoxification, current study was designed to better characterize expression coho salmon (Oncorhynchus kisutch) effects salinity acclimation on those enzymes. Livers, gills, olfactory tissues were collected from (O. after they had undergone freshwater various regimes seawater (8, 16 32 g/L). Using immunoblot techniques coupled with testosterone hydroxylase catalytic activities, 4 orthologs cytochrome P450 (CYP1A, CYP2K1, CYP2M1, CYP3A27) measured each tissue. Also, 2 transcripts flavin-containing monooxygenases (FMO A B) associated activities measured. With exception CYP1A, which down-regulated liver, protein other 3 induced at higher salinity, greatest increase observed CYP2M1 tissues. In liver 6β- 16β-hydroxylation also significantly increased hypersaline acclimation. Similarly, FMO up-regulated all a salinity-dependent pattern, whereas B mRNA down-regulated. FMO-catalyzed benzydamine N-oxygenase methyl p-tolyl sulfoxidation gills hypersalinity, but either unchanged or not detected These data demonstrate that conditions may alter toxicity chemicals during freshwater/saltwater