Feruloylation and structure of arabinoxylan in wheat endosperm cell walls from RNAi lines with suppression of genes responsible for backbone synthesis and decoration.

摘要: Arabinoxylan (AX) is the major component of cell walls wheat grain (70% in starchy endosperm), an important determinant end-use qualities affecting food processing, use for animal feed and distilling a source dietary fibre human diet. AX heterogeneous polysaccharide composed fractions which can be sequentially extracted by water (WE-AX), then xylanase action (XE-AX) leaving unextractable (XU-AX) fraction. We determined arabinosylation feruloylation these both wild-type RNAi lines with decreased content (TaGT43_2 RNAi, TaGT47_2 RNAi) or arabinose 3-linked to mono-substituted xylose (TaXAT1 RNAi). show that are characterized degree AX, <5, 5-7 13-19 mg bound ferulate (g-1 AX), their diferulates (diFA), <0.3, 1-1.7 4-5 mg WE, XE XU fractions, respectively, all control lines. The amount its were less affected transgenes XE-AX fraction than WE-AX largely unaffected XU-AX As majority diFA associated fraction, there was only small effect no on total content. Our results compatible model where, maintain wall function, maintained at stable levels when other properties altered.