Vascular tropism models of blood-borne microbial dissemination

摘要: Similar to circulating tumour and immune cells, many blood-borne microbes preferentially 9home9 specific vascular sites tissues during hematogenous dissemination 1-5. For pathogens, the 9postal codes9 mechanisms responsible for tissue-specific tropism are unknown have been challenging unravel. Members of Lyme disease Borreliella burgdorferi species complex infect a broad range mammalian exhibit strain-, species- host-specific tissue patterns. Intravenous perfusion experiments intravital microscopy studies suggest that heterogeneous properties may depend on differences in host microbial molecules supporting interaction extravasation. However, interpreting these can be complicated because immune-protective moonlighting (multitasking) B. adhesins. Here, we investigated whether measured by live cell imaging particle tracking aorta, bladder, brain, joint skin microvascular flow chamber models predict strain- patterns vivo These identified endothelial type-specific accurately predicted but not indicated all distinct. Thus, ability interact with surfaces under physiological shear stress is key determinant bacteria. The methods model systems reported here will invaluable identifying characterizing diverse, largely undefined useful studying other microbes.