Immobilization of lipase by adsorption on a particulate macroporous resin

作者: Peter Eigtved

DOI:

关键词: ParticulatesParticle sizeAdsorptionPseudomonasMaterials scienceSurface areaChromatographyCandida antarcticaLipaseInteresterified fat

摘要: Lipase is immobilized by adsorption on a polymethacrylic acid ester resin such as polymethylmethacrylate cross-linked with divinyl benzene. The preferably particulate, macroporous having an average pore radius of 100-200 Å, total surface area 25-150 m 2 /g and particle size 100-1,000 μm. lipase may be obtained from Mucor miehei, Candida antarctica, Pseudomonas cepacia or Humicola lanuginosa. dried used for interesterifying ester, hydrolyzing synthesizing ester. Interesterification can carried out continuously in fixed-bed column.

参考文章(4)
Takaharu Matsuo, Norio Sawamura, Wataru Hashida, Yukio Hashimoto, Method for enzymatic transesterification of lipid and enzyme used therein ,(1981)
Hidetoshi Kutsuki, Kiyoshi Watanabe, Takehisa Ohashi, Yoshio Shimada, Kenji Takahara, Hideyuki Takahashi, Yoshio Nakamura, Shigaki Hamaguchi, Masanori Asada, Process for preparing optically active indoline-2-carboxylic acid ,(1986)