Method and apparatus for automated identification and enumeration of specified blood cell subclasses

摘要: Specified subclasses of blood cells, such as lymphocytes, are automatically identified based on utilization antigenic determinants the cell surface, their reactivity with antibodies which fluoresce under known circumstances, and principles flow cytometry. A sample is first incubated a reagent including to lymphocyte subclass be identified, being directly or indirectly made fluorescently responsive particular light (e. g. argon ion laser). The illuminated, at time, by focused coherent light, forward scatter, right angle fluorescence detected used identify enumerate cells specified subclass.