Invalidity of the acetylene reduction assay in alkane-utilizing, nitrogen-fixing bacteria.

作者： J A De Bont , E G Mulder

DOI: 10.1128/AEM.31.5.640-647.1976

关键词: Ethylene 、 Organic chemistry 、 Anaerobic oxidation of methane 、 Nitrogenase 、 Photochemistry 、 Bacteria 、 Methane 、 Alkane 、 Methylococcaceae 、 Acetylene 、 Chemistry 、 Biotechnology 、 Food science 、 Ecology (disciplines) 、 Applied Microbiology and Biotechnology

摘要: The cause of the failure C2H2-C2H4 assay for nitrogen-fixing bacteria growing on lower alkanes was studied. Acetylene a strong competitive inhibitor methane oxidation methane-utilizing bacteria, as well by other so that energy and reducing power were no longer available reduction acetylene nitrogenase. Nitrogen-fixing grown may reduce when intermediates alkane-breakdown or substrates oxidizable in presence are supplied. Ethylene co-oxidation is not responsible test, because also inhibits this along with oxidation.