Characterization of the human granulocyte-macrophage colony-stimulating factor receptor.

摘要: Human granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine derived from activated T cells, endothelial fibroblasts, and macrophages. It stimulates myeloid erythroid progenitors to form colonies in semisolid medium vitro, as well enhancing multiple differentiated functions of mature neutrophils, macrophages, eosinophils. We have examined the binding human GM-CSF variety responsive cells cell lines. The most myelomonocytic specifically eosinophils, express highest numbers single class high affinity receptors (Kd approximately 37 pM, 293-1000 sites/cell). HL-60 KG-1 exhibit an increase specific at concentrations GM-CSF; computer analysis data nonetheless consistent with sites Kd 43 pM 20-450 sites/cell. Dimethyl sulfoxide induces 3-10-fold expressed coincident terminal neutrophilic differentiation. Finally, 125I-GM-CSF fresh peripheral blood six patients chronic myelogenous leukemia was analyzed. In three cases, similar nonsaturable observed cells. low, or some undetectable on myeloblasts obtained eight acute leukemia. affinities receptor for are all known biological activities. Affinity labeling both normal neutrophils dimethyl sulfoxide-induced unglycosylated yielded band 98 kDa, implying molecular weight 84,000 receptor.