Interactions between Hepatitis C Virus and the Human Apolipoprotein H Acute Phase Protein: A Tool for a Sensitive Detection of the Virus

摘要: The Hepatitis C virus (HCV) infection exhibits a high global prevalence frequently associated with hepatocellular carcinoma, taking years to develop. Despite the standardization of highly sensitive HCV quantitative RT-PCR (qRT-PCR) detection methods, false-negative diagnoses may be generated current mainly due presence PCR inhibitors and/or low viral loads in patient's sample. These impact both public health systems, developing countries, and an lesser extent, developed including risk transmission during organ transplantation blood transfusion quality antiviral treatment monitoring. To adopt appropriate therapeutic strategy improve prognosis, it is urgent increase sensitivity. Based upon previous studies on HBV, we worked capacity scavenger acute phase protein, Apolipoprotein H (ApoH) interact HCV. Using different approaches, immunoassays, antibody-inhibition, oxidation, ultracentrifugation, electron microscopy analyses, demonstrated specific interactions between particles ApoH. Moreover, when using two-step process, capture by ApoH-coated nanomagnetic beads home-made real-time HCV-RT-PCR, confirmed for all samples from clinical collection HCV-seropositive patients exhibiting COBAS® TaqMan® Test, v2.0 (COBAS)-positive result. In contrast, either HCV-load as determined COBAS or HCV-negative results, addition ApoH-HCV-capture HCV-detection process was able sensitivity more interestingly, detect genotype sequence-independent manner, high-proportion (44%) HCV/RNA-positive among patients. Thus, immune interaction ApoH could used sample preparation tool enrich cleanse enhance therefore significantly reduce numbers diagnosis results.