An ELISA optimized for porcine epidemic diarrhoea virus detection in faeces.
作者： L. Rodák , L. Valíček , B. Šmíd , Z. Nevoránková
DOI: 10.1016/J.VETMIC.2004.09.020
关键词: Coronavirus 、 Rotavirus 、 Biology 、 Feces 、 Antibody 、 Nidovirales 、 Virus 、 Virology 、 Coronaviridae 、 Microbiology 、 Porcine epidemic diarrhea virus
摘要: Monoclonal antibodies to porcine epidemic diarrhoea virus (PEDV) membrane protein M were prepared and used for the comparative assessment of three blocking ELISA variants detect PEDV. The competitive (CB-ELISA) format showed highest sensitivity, allowing detection 10(2.5) plaque-forming units PEDV/ml in culture medium. Its specificity was verified by inclusion control samples containing transmissible gastroenteritis (TGEV) rotavirus A each analysis. Eighty field faeces obtained from 38 herds affected with examined, PEDV found 15 (19%) 6 (16%) herds. suitability CB-ELISA screening epizootiologic situations is discussed.
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