Vitellogenin synthesis by the fat body of the mosquito Aedes aegypti

摘要: Synthesis of vitellogenin (yolk protein) by the fat body Aedes aegypti is triggered blood meal. Total RNA begins to rise 2 hr post meal (PBM) and increases 3-fold 12 hr. Vitellogenin synthesis detectable 3–4 PBM, reaches a peak 28 PBM. After PBM both total ability synthesize vitellogenins fall precipitously. Actinomycin D at 10 μg/ml inhibits about 90%, but does not inhibit in vitro tissue proteins. At this concentration actinomycin, remains constant for up 6 hr, suggesting presence relatively long-lived messenger RNA. When injected into mosquitoes, actinomycin prevents normal increase rate allows proceed occurring time injection. The results suggest that triggers ribosomal necessary later synthesis.