Quantitative Analysis of Ubiquitinated Proteins in Human Pituitary and Pituitary Adenoma Tissues.
作者： Shehua Qian , Xiaohan Zhan , Miaolong Lu , Na Li , Ying Long
关键词: Western blot 、 Proteomic Profiling 、 Hippo signaling pathway 、 Biology 、 Signal transduction 、 Nucleotide excision repair 、 Quantitative proteomics 、 Ubiquitin 、 Cell biology 、 Protein ubiquitination
摘要: Protein ubiquitination is an important post-translational modification that associated with multiple diseases, including pituitary adenomas (PAs). profiling in human and PAs remains unknown. Here, we performed the first analysis anti-ubiquitin antibody (specific to K-e-GG)-based label-free quantitative proteomics method bioinformatics investigate protein between PA control tissues. A total of 158 ubiquitinated sites 142 peptides 108 proteins were identified, five motifs found. KEGG pathway network identified four statistically significant signaling pathways, PI3K-AKT pathway, hippo ribosome, nucleotide excision repair. R software Gene Ontology (GO) revealed was involved biological processes, cellular components, molecule functions. The randomly selected 14-3-3 zeta/delta further analyzed Western blot, it found upregulated nonfunctional might be derived from significantly decreased level its compared pituitaries, which indicated a contribution tumorigenesis. These findings provided proteomic ubiquitination-involved networks PAs. This study offers new scientific evidence basic data elucidate functions PAs, insights into novel molecular mechanisms tumorigenesis, discovery biomarkers therapeutic targets for effective treatment
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