Examination of colonies and stool blots for detection of enteropathogens by DNA hybridization with eight DNA probes.

摘要: We compared three methods for detecting enteropathogens in 416 children with diarrhea: (i) examination of 10 lactose-fermenting and all non-lactose-fermenting Escherichia coli (colony blots); (ii) 300 colonies (replicate (iii) determination the total bacterial growth stools (stool blots). All specimens were spotted onto Whatman 541 filters hybridized specific radiolabeled DNA probes. Enterotoxigenic E. was detected 38 patients by examining colony blots, 52 replicate 45 stool blots. Enteropathogenic adhesin factor 12 25 16 that enterohemorrhagic probe 2 11 0 Shiga-like toxin-producing Shigella spp. identified standard bacteriological 82 patients, enteroinvasive blots (total, 93), 56 35 patients. Of culture-confirmed infections, 17-kilobase-pair 36 Ipa (P less than 0.05). Examining probes more enterotoxigenic E.coli < 0.005), enteropathogenic adhesion factor-producing 0.001), 0.005) infections More a 0.001).