Folate chitosan conjugated doxorubicin and pyropheophorbide acid nanoparticles (FCDP–NPs) for enhance photodynamic therapy

摘要: We prepared new folate chitosan conjugated doxorubicin (DOX) and pyropheophorbide acid (PPa) nanoparticles (FCDP–NPs) using an ionic gelation method with tripolyphosphate (TPP) to enhance photodynamic therapy activity, based on the considerations of long absorption wavelength (683 nm) in water excellent chemotherapeutic characteristics cancer therapy. The obtained FCDP–NPs demonstrated a typical spherosome structure, strong near infrared (NIR) (705 significantly improved stability dispersity PBS (pH = 5, 7, 9); as well high singlet oxygen quantum yield (ΦΔ 64%) compared free PPa 59.1%). In addition, vitro cell experiments suggested that could be uptaken by HepG2 cells quickly were mainly located nucleus. showed PDT efficiency over pure DOX at same concentration after irradiation. Specifically, lead 92% inhibition rate 40 μg mL−1 (equal 6 DOX). However, little cytotoxicity mL−1, which suggests small amount effectively activities “dark” cytotoxicity. Moreover, morphological changes treatment further indicated induce damage apoptotic death efficiently. Finally, photochemical mechanism during process was investigated specific quenching agents sodium azide (SA, single quencher) D-mannitol (DM, hydroxyl radicals quencher), respectively. results Type I II reactions can occur simultaneously, yet reaction (the generation radicals) might play more important role. All these studies potential treatment.