MicroRNA profiling of ovarian granulosa cell tumours reveals novel diagnostic and prognostic markers.

摘要: The aim of this study was to explore the clinical utility microRNAs (miRNAs) as improved markers ovarian granulosa cell tumours (GCTs) for cancer diagnosis and prognosis prediction. Current histopathological genetic markers, such presence a FOXL2 gene mutation distinguish between two major subtypes are not wholly accurate novel biomarkers warranted. miRNA expression profiles five formalin-fixed, paraffin-embedded (FFPE) adult-GCTs juvenile-GCTs were assessed using Affymetrix 3.0 Arrays compared differential expression. Ten miRNAs in an additional 33 FFPE four normal samples by quantitative RT-PCR, their correlated information. MicroRNA array found 37 differentially expressed GCT (p < 0.05, fold change ≥2 among these, miRs -138-5p, -184, -204-5p, -29c-3p, -328-3p -501-3p validated RT-qPCR (p < 0.05). In addition, miR-184 predictive tumour recurrence adult-GCTs, specifically patients diagnosed with stage I II only disease (p < 0.001 p < 0.05, respectively). This is first report on global human GCTs samples. We have six subtype classification low levels miR-138-5p correlating early stage. Low abundance adult-GCT candidate biomarker. Further studies now needed confirm these diagnostic understand possible roles pathogenesis GCTs.