GPD1, which encodes glycerol-3-phosphate dehydrogenase, is essential for growth under osmotic stress in Saccharomyces cerevisiae, and its expression is regulated by the high-osmolarity glycerol response pathway.

摘要: The yeast Saccharomyces cerevisiae responds to osmotic stress, i.e., an increase in osmolarity of the growth medium, by enhanced production and intracellular accumulation glycerol as a compatible solute. We have cloned gene encoding key enzyme synthesis, NADH-dependent cytosolic glycerol-3-phosphate dehydrogenase, we named it GPD1. gpd1 delta mutants produced very little glycerol, they were sensitive stress. Thus, is indeed essential for cells during reduced water availability. hog1 lacking protein kinase involved osmostress-induced signal transduction (the high-osmolarity response [HOG] pathway) failed dehydrogenase activity mRNA levels when stress was imposed. expression GPD1 regulated through HOG pathway. However, there may be Hog1-independent mechanisms mediating accumulation, since strain could still enhance its content, although less than wild type. are more isogenic strains, double even either single mutant. pathway most probably has additional targets mechanism adaptation hypertonic medium.