Association between NPPA promoter methylation and hypertension: results from Gusu cohort and replication in an independent sample.

摘要: Atrial natriuretic peptide (ANP), one of the main members peptides system, has been associated with hypertension and related complications, but underlying molecular mechanisms are not very clear. Here, we aimed to examine whether DNA methylation, a modification genome, A gene (NPPA), coding ANP, was hypertension. Peripheral blood methylation NPPA promoter quantified by target bisulfite sequencing in 2498 community (mean aged 53 years, 38% men) as discovery sample 1771 independent participants 62 years, 54% replication sample. In both samples, conducted single CpG association analysis, followed gene-based between hypertension, adjusting for age, sex, education level, cigarette smoking, alcohol consumption, obesity, fasting glucose, lipids. Multiple testing controlled false rate approach. Of 9 loci assayed, hypermethylation at 5 CpGs (CpG1, CpG3, CpG6, CpG8, CpG9) significantly lower odds prevalent sample, (CpG1 located Chr1:11908353) successfully replicated (OR = 0.82, 95%CI 0.74–0.91, q 0.002) after covariates multiple testing. The analysis found that whole pressure samples (all P < 0.05). levels were decreased Chinese adults Aberrant may participate