β-Carotene 15,15′-monooxygenase 1 single nucleotide polymorphisms in relation to plasma carotenoid and retinol concentrations in women of European descent

摘要: Background: Carotenoids have been hypothesized to reduce the risk of many diseases, but associations with intakes or blood concentrations may arise from other constituents fruit and vegetables. Use genetic variation in β-carotene 15,15′-monooxygenase 1 (BCMO1), a key enzyme provitamin A carotenoid metabolism, as surrogate for exposure aid determining role carotenoids unconfounded by carotenoid-containing food constituents, important variants must be identified. Objective: Our goal was select BCMO1 single nucleotide polymorphisms (SNPs) that predict plasma use future epidemiologic studies. Design: We assessed between 224 SNPs ± 20 kb imputed 1000 Genomes Project EUR reference panel retinol using 7 case-control data sets (n = 2344) within Nurses’ Health Study, randomly divided into training 1563) testing 781) sets. were chosen set through stepwise selection multivariate linear regression models; β-coefficients used weights weighted gene scores. Results: Two 3 selected predictors β-carotene, α-carotene, β-cryptoxanthin, lutein/zeaxanthin. In set, scores significantly associated corresponding (P 6.4 × 10−12, 3.3 10−3, 0.02, 1.8 10−17, respectively), differed 48%, 15%, 36%, respectively, across extreme score quintiles. Conclusions: are concentrations. Given adequate sample size, useful surrogates studies.