Vector status of three leafhopper species for Australian lucerne yellows phytoplasma
作者： Leigh J Pilkington , Geoff M Gurr , Murray J Fletcher , Alex Nikandrow , Eric Elliott
DOI: 10.1111/J.1440-6055.2004.00419.X
关键词: Horticulture 、 Botany 、 Batracomorphus angustatus 、 Vector (epidemiology) 、 Phytoplasma 、 Pathogen 、 Orosius argentatus 、 Leafhopper 、 Polymerase chain reaction 、 Biology 、 Restriction fragment length polymorphism
摘要: The leafhoppers Orosius argentatus (Evans), Austroagallia torrida (Evans) and Batracomorphus angustatus (Osborn) were used in transmission tests to determine their vector status for the phytoplasma associated with Australian lucerne yellows (ALuY). Caged, seed-grown plants monitored foliar symptom expression after feeding by transferred from ALuY symptomatic plants. Twelve of 25 developed disease-like symptoms including stunting yellowing. most pronounced displayed five that had been fed on O. argentatus four A. torrida. One plant, O. argentatus, showed distinctive root ALuY. A was identified electron microscopy two one For each group a single leafhopper species, plant positive as determined polymerase chain reaction (PCR) using universal primers. detected PCR A. torrida restriction fragment length polymorphism (RFLP) analysis tomato big bud (TBB) phytoplasma. product B. angustatus too faint RFLP analysis. assays conducted DNA extracted head thorax species field-collected insects, but no detected. These findings suggest is pathogen TBB
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