Detection of guanyl cyclase in mammalian tissues.
作者： Arnold A. White , G.D. Aurbach
DOI: 10.1016/0005-2744(69)90362-3
关键词: Sucrose 、 Spleen 、 GTP' 、 Enzyme 、 Biochemistry 、 GUCY1B3 、 Biology 、 Guanylate cyclase 、 Kidney 、 GUCY1A3
摘要: Abstract Guanyl cyclase was assayed by determining the rate of conversion [ α - 32 P ]GTP to cyclic 3′,5′-[ P]GMP. A two-step chromatographic procedure developed for isolating product. The enzyme found in soluble fraction after homogenizing tissues 0.3 M sucrose. reaction showed a pH optimum near 7.6 and dependent on Mn 2+ . relative enzymatic activity rat lung >> brain = spleen > kidney liver heart.
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