Controlled release of raloxifene by nanoencapsulation: effect on in vitro antiproliferative activity of human breast cancer cells

摘要: Raloxifene hydrochloride (RH) is considered to be an antiproliferative agent of mammary tissue. The aim this study was investigate the effect encapsulation RH in polymeric nanocapsules with anionic or cationic surface on its release profile and activity. They were prepared by interfacial deposition preformed polymer, followed wide physicochemical characterization. vitro assessed dialysis membrane method data analyzed mathematical modeling. MCF-7 cell viability investigated 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay as well counting viable cells. had high efficiency, low polydispersity, nanometric mean size. Nanocapsules Eudragit(®) RS100 S100 presented positive negative zeta potentials, respectively. Drug studies demonstrated controlled from nanocapsules, which could explained due a stronger interaction drug these larger amount entrapped drug. On other hand, control not observed adsorbed onto their surface. showed that RH-loaded promote best activity after 24 hours treatment, whereas for 72 hours. Furthermore, combined treatment formulations improved during entire treatment.