Plasma exosomes from depression ameliorate inflammation-induced depressive-like behaviors via sigma-1 receptor delivery.

摘要: A growing body of studies indicated that exosomes are one vital players in pathological process neuropsychiatric diseases, but their role major depressive disorder (MDD) remains poorly understood. Here we purified plasma from depression including lipopolysaccharide (LPS)-challenged depression, chronic restraint stress (CRS)-induced MDD subjects, and control mice or volunteers. The therapeutic effect these exogenous was assessed utilizing behavioral tests biochemical approaches the LPS-caused microglial BV2 cells. expression exosomal sigma-1 receptor (Sig-1R) evaluated by western blotting. Sig-1R biological function determined using knockout HEK 293 Our results revealed injection models patients rather than normal controls significantly ameliorated depressive-like behaviors, deficiency BDNF neuro-inflammation LPS-challenged mice. In addition, co-culture with instead prevented LPS-induced inflammation responses Moreover, demonstrated for first time to be enriched compared controls. However, null no longer emerged antidepressant-like action Thus, exerted effects a dependent manner depression. This work improves our understanding suggesting novel exosomes-based approach treatment.