Products of the porcine group C rotavirus NSP3 gene bind specifically to double-stranded RNA and inhibit activation of the interferon-induced protein kinase PKR.

摘要: The porcine group C rotavirus (Cowden strain) NSP3 protein (the equivalent of the A gene 7 product, formerly called NS34) shares homology with known double-stranded RNA-binding proteins, such as interferon-induced, RNA-dependent kinase PKR. clone NSP3, expressed both in vitro and COS-1 cells, led to synthesis minor amounts a product an M(r) 45,000 expected full-length NSP3) major products M(r)s 38,000 8,000. Restriction enzyme digestion analysis prior expression amino-terminal sequence suggest that 8,000 are cleavage 45,000. 8,000, which contain motif present bound specifically RNA. were also detected Cowden strain-infected MA104 cells. cells capable inhibiting activation similar other HeLa rescuing replication interferon-sensitive deletion mutant vaccinia virus.