Rapid Detection of West Nile Virus from Human Clinical Specimens, Field-Collected Mosquitoes, and Avian Samples by a TaqMan Reverse Transcriptase-PCR Assay

摘要: The authors report on the development and application of a rapid TaqMan assay for detection West Nile (WN) virus in variety human clinical specimens field-collected specimens. Oligonucleotide primers FAM- TAMRA-labeled WN virus-specific probes were designed by using nucleotide sequence New York 1999 isolate. was compared to traditional reverse transcriptase (RT)-PCR isolation Vero cells with large number ( approximately 500) obtained from humans (serum, cerebrospinal fluid, brain tissue), mosquitoes, avian tissue samples. specific demonstrated greater sensitivity than RT-PCR method correctly identified 100% culture-positive mosquito pools 98% should be utility diagnostic laboratory complement existing testing as tool conduct surveillance United States.