Isolation of C. elegans and related nematodes.

摘要: Isolating Caenorhabditis and other nematodes from the wild first requires field sampling (reviewed in Section 1). The easiest most efficient way to recover animals any substrate is place sample onto a standard C. elegans culture plate (Section 2.1). Alternative methods used by nematologists soil (Sections 2.2, 2.3, 2.4) are our hands more difficult implement only yield fraction of individuals sample. A tricky step recognize your species interest out zoo nematode that comes with typical 3). Culture 4) freezing 5) conditions then reviewed. Finally, we briefly summarize organization timing an isolation experiment 6), as well available collections 7). Bear mind this chapter strongly focused towards close relatives.