作者: J. C. Lewis , N. L. Jones , W. G. Jerome
DOI:
关键词: Cytochemistry 、 Very low-density lipoprotein 、 Acid phosphatase 、 Cytoplasm 、 Foam cell 、 Endoplasmic reticulum 、 Lipoprotein 、 Biology 、 Cell biology 、 Monocyte
摘要: Lysosomes have long been implicated as a factor contributing to the progression and complication of atherosclerosis. The authors' laboratory previously has shown that lysosomal ultrastructure in arterial macrophage foam cells is altered primary lysosomes give rise large pleiomorphic organelles on lipid accumulation during lesion progression. To further explore subcellular alterations associated cell formation, three-dimensional (3D) intermediate voltage electron microscopy was used examine monocyte-derived macrophages (monocyte/macrophages) early vitro uptake beta migrating very-low-density lipoproteins (beta VLDL). were identified using acid phosphatase cytochemistry, control these constituted 3.5% total cytoplasmic volume. Both secondary observed. Upon VLDL uptake, volume acid-phosphatase-positive increased threefold over 30 minutes, reaction product found three additional morphologically distinct structures: tubular lysosomes, membrane stacks, endoplasmic reticulum with widened cisternae. proportion occupied by each five quantitated at 10 1 hour, 4 hours incubation, their relative abundance compared controls processed either no lipoprotein challenge or albumin incubation for hour. Secondary compartment peaked minutes; ensuing 3.5 hours, however, progressively shifted new membrane-limited locations. Our observations document complex 3D organization spacial relationships among structures induced uptake. patterns lipoprotein-stimulated pigeon monocyte/macrophages similar several aspects observed lesions.