Duck hepatitis B photoinactivation bydimethylmethylene blue in RBC suspensions.
作者： Stephen J. Wagner , Andrey Skripchenko , John C. Pugh , Donna B. Suchmann , M. Khalid Ijaz
DOI: 10.1046/J.1537-2995.2001.41091154.X
关键词: Hepadnaviridae 、 Virology 、 In vitro 、 Virus 、 Biology 、 Avihepadnavirus 、 Incubation 、 Methylene blue 、 Virus quantification 、 Duck hepatitis B virus
摘要: BACKGROUND: Dimethylmethylene blue (DMMB) has been used to photoinactivate a number of model viruses, including VSV, in RBC suspensions under conditions that preserve vitro properties during storage.23 The relative sensitivity duck HBV (DHBV) and VSV photoinactivation by DMMB was investigated performing an indirect immunofluorescence assay (IFA) using primary hepatocyte (PDH) cultures or standard plaque for the respective viruses. STUDY DESIGN AND METHODS: added 45-percent Hct, WBC-reduced, oxygenated AS-3 RBCs at 10-, 1-, 0.1-μM concentrations. Samples (1-mm thick) were illuminated with 5.4-mW per cm2 red light 2 9 seconds. Unilluminated samples without 10 μM served as control. RESULTS: DHBV rapidly photoinactivated concentration light-dose-dependent fashion. Neither virus substantially inactivated incubation dark. For given exposure, required one-one hundredth achieve approximately same level inactivation. CONCLUSION: appears be considerably more sensitive than photoinactivation. Photoinactivation Hct can achieved seconds micromolar quantities dye.
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