Scanning-fluorescence Reader Based on Embedded System

作者: Zhonglong Zhao , Xiaoping Min , Shengxiang Ge , Ningshao Xia

DOI: 10.12928/TELKOMNIKA.V13I3.1808

关键词: OptoelectronicsWaveformSmoothingBeam (structure)LinearityMaterials scienceComputer hardwareSignalDiodeOptical fiberFluorescence

摘要: To measure the concentration of C-reactive protein (CRP) in serum, a portable, scanning-fluorescence reader based on time-resolved fluoroimmunoassays was developed. The integrates with AD7707 converter, which performs at high accuracy. photosensitive diode acts as photoelectric conversion device, an optical module fibers, is able to concentrate excitation light from LED into line-shape beam, designed sendand receive signal. device subsequently addresses waveform data using gradient, smoothing, and binarization method. When measures CRP fluorescence test strip, results exhibited good linearity (0.99998) CVs (coefficient variation) were below 5%, indicate At same time system low cost small size.

参考文章(20)
A. Handa-Corrigan, S. Nikolay, D. Jeffery, B. Heffernan, A. Young, Controlling and predicting monoclonal antibody production in hollow-fiber bioreactors. Enzyme and Microbial Technology. ,vol. 14, pp. 58- 63 ,(1992) , 10.1016/0141-0229(92)90027-L
Wingman Leung, Cangel Puiyee Chan, Timothy H Rainer, Margaret Ip, George WH Cautherley, Reinhard Renneberg, None, InfectCheck CRP barcode-style lateral flow assay for semi-quantitative detection of C-reactive protein in distinguishing between bacterial and viral infections Journal of Immunological Methods. ,vol. 336, pp. 30- 36 ,(2008) , 10.1016/J.JIM.2008.03.009
Terry W Du Clos, Function of C-reactive protein. Annals of Medicine. ,vol. 32, pp. 274- 278 ,(2000) , 10.3109/07853890009011772
Xianbo Qiu, Changchun Liu, Michael G. Mauk, Robert W. Hart, Dafeng Chen, Jing Qiu, Terry Kientz, Jonathan Fiene, Haim H. Bau, A portable analyzer for pouch-actuated, immunoassay cassettes Sensors and Actuators B-chemical. ,vol. 160, pp. 1529- 1535 ,(2011) , 10.1016/J.SNB.2011.08.012
Shuo Wang, Can Zhang, Junping Wang, Yan Zhang, None, Development of colloidal gold-based flow-through and lateral-flow immunoassays for the rapid detection of the insecticide carbaryl Analytica Chimica Acta. ,vol. 546, pp. 161- 166 ,(2005) , 10.1016/J.ACA.2005.04.088
Ming Li, Enze Sheng, Yulong Yuan, Xiaofeng Liu, Xiude Hua, Minghua Wang, Sensitive time-resolved fluoroimmunoassay for quantitative determination of clothianidin in agricultural samples Environmental Science and Pollution Research. ,vol. 21, pp. 5803- 5809 ,(2014) , 10.1007/S11356-014-2506-7
Zhongqiang Yan, Lei Zhou, Yongkai Zhao, Jing Wang, Lihua Huang, Kongxin Hu, Haihong Liu, Hong Wang, Zhaobiao Guo, Yajun Song, Huijie Huang, Ruifu Yang, Rapid quantitative detection of Yersinia pestis by lateral-flow immunoassay and up-converting phosphor technology-based biosensor. Sensors and Actuators B-chemical. ,vol. 119, pp. 656- 663 ,(2006) , 10.1016/J.SNB.2006.01.029
Tian-Cai Liu, Mei-Jun Chen, Zhi-Qi Ren, Jing-Yuan Hou, Guan-Feng Lin, Ying-Song Wu, Development of an improved time-resolved fluoroimmunoassay for simultaneous quantification of C-peptide and insulin in human serum Clinical Biochemistry. ,vol. 47, pp. 439- 444 ,(2014) , 10.1016/J.CLINBIOCHEM.2014.01.016
Peter B. Luppa, Carolin Müller, Alice Schlichtiger, Harald Schlebusch, Point-of-care testing (POCT): Current techniques and future perspectives. Trends in Analytical Chemistry. ,vol. 30, pp. 887- 898 ,(2011) , 10.1016/J.TRAC.2011.01.019
John Danesh, Jeremy G. Wheeler, Gideon M. Hirschfield, Shinichi Eda, Gudny Eiriksdottir, Ann Rumley, Gordon D.O. Lowe, Mark B. Pepys, Vilmundur Gudnason, C-Reactive Protein and Other Circulating Markers of Inflammation in the Prediction of Coronary Heart Disease The New England Journal of Medicine. ,vol. 350, pp. 1387- 1397 ,(2004) , 10.1056/NEJMOA032804