miR-133a regulates vitamin K 2,3-epoxide reductase complex subunit 1 (VKORC1), a key protein in the vitamin K cycle.
作者： Virginia Pérez-Andreu , Raúl Teruel , Javier Corral , Vanessa Roldán , Nuria García-Barberá
DOI: 10.2119/MOLMED.2012.00062
关键词: Vitamin K epoxide reductase 、 Regulation of gene expression 、 Transfection 、 microRNA 、 Biology 、 Three prime untranslated region 、 VKORC1 、 Reductase 、 Protein subunit 、 Molecular biology
摘要: Regulation of key proteins by microRNAs (miRNAs) is an emergent field in biomedicine. Vitamin K 2,3-epoxide reductase complex subunit 1 (VKORC1) a relevant molecule for cardiovascular diseases, since it the target oral anticoagulant drugs and plays role soft tissue calcification. The objective this study was to determine influence miRNAs on expression VKORC1. Potential targeting VKORC1 mRNA were searched using online algorithms. Validation studies carried out HepG2 cells miRNA precursors; direct interaction investigated with reporter assays. In silicostudies identified two putative conserved binding sites miR-133a miR-137 mRNA. Ex vivo showed that only expressed liver; transfection precursors reduced dose-dependent manner, as assessed quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) well protein expression. Reporter assays HEK293T interacts 3′UTR Additionally, levels correlated inversely 23 liver samples from healthy subjects. conclusion, appears have regulatory effect humans; regulation may potential importance therapy or aortic
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