RNAi screening of human glycogene orthologs in the nematode Caenorhabditis elegans and the construction of the C. elegans glycogene database.
作者： Sayaka Akiyoshi , Kazuko H Nomura , Katsufumi Dejima , Daisuke Murata , Ayako Matsuda
关键词: Cell biology 、 Database 、 Genetics 、 Phenotype 、 RNA interference 、 Embryonic stem cell 、 Caenorhabditis elegans 、 Mitosis 、 Germline 、 Cell cycle 、 Cell Cycle Protein 、 Biology
摘要: In this study, we selected 181 nematode glycogenes that are orthologous to human and examined their RNAi phenotypes. The results deposited in the Caenorhabditis elegans Glycogene Database (CGGDB) at AIST, Tsukuba, Japan. most prominent phenotypes observed disruptions of cell cycle progression germline mitosis/meiosis early embryonic mitosis. Along with previously reported roles chondroitin proteoglycans, glycosphingolipids GPI-anchored proteins progression, show for first time inhibition functions N-glycan synthesis genes (cytoplasmic alg genes) resulted abnormal formation, ER stress small body size provide additional information on glycoconjugates mechanisms cells.
elsevier.com
oxfordjournals.org
oup.com
sci-hub.se 参考文章(65)
Eillen Tecle, Carlos A. Diaz-Balzac, Hannes E. Bülow, Distinct 3-O-sulfated heparan sulfate modification patterns are required for kal-1-dependent neurite branching in a context-dependent manner in Caenorhabditis elegans. G3: Genes, Genomes, Genetics. ,vol. 3, pp. 541- 552 ,(2013) , 10.1534/G3.112.005199
Andrea K. Kalis, Mary B. Kroetz, Kathleen M. Larson, David Zarkower, Functional genomic identification of genes required for male gonadal differentiation in Caenorhabditis elegans. Genetics. ,vol. 185, pp. 523- 535 ,(2010) , 10.1534/GENETICS.110.116038
E. Marza, K. T. Simonsen, N. J. Faergeman, G. M. Lesa, Expression of ceramide glucosyltransferases, which are essential for glycosphingolipid synthesis, is only required in a small subset of C. elegans cells Journal of Cell Science. ,vol. 122, pp. 822- 833 ,(2009) , 10.1242/JCS.042754
Donald L. Court, Andrew Fire, Lisa Timmons, Ingestion of bacterially expressed dsRNAs can produce specific and potent genetic interference in Caenorhabditis elegans Gene. ,vol. 263, pp. 103- 112 ,(2001) , 10.1016/S0378-1119(00)00579-5
Lena M Kutscher, Shai Shaham, Forward and reverse mutagenesis in C. elegans WormBook. pp. 1- 26 ,(2014) , 10.1895/WORMBOOK.1.167.1
Katsufumi Dejima, Akira Seko, Katsuko Yamashita, Keiko Gengyo-Ando, Shohei Mitani, Tomomi Izumikawa, Hiroshi Kitagawa, Kazuyuki Sugahara, Souhei Mizuguchi, Kazuya Nomura, Essential Roles of 3′-Phosphoadenosine 5′-Phoshosulfate Synthase in Embryonic and Larval Development of the Nematode Caenorhabditis elegans Journal of Biological Chemistry. ,vol. 281, pp. 11431- 11440 ,(2006) , 10.1074/JBC.M601509200
Katsufumi Dejima, Daisuke Murata, Souhei Mizuguchi, Kazuko H. Nomura, Tomomi Izumikawa, Hiroshi Kitagawa, Keiko Gengyo-Ando, Sawako Yoshina, Tomomi Ichimiya, Shoko Nishihara, Shohei Mitani, Kazuya Nomura, Two Golgi-resident 3′-Phosphoadenosine 5′-Phosphosulfate Transporters Play Distinct Roles in Heparan Sulfate Modifications and Embryonic and Larval Development in Caenorhabditis elegans Journal of Biological Chemistry. ,vol. 285, pp. 24717- 24728 ,(2010) , 10.1074/JBC.M109.088229
Patricia Berninsone, Carbohydrates and glycosylation Wormbook. pp. 1- 22 ,(2006) , 10.1895/WORMBOOK.1.125.1
The structure of the nervous system of the nematode Caenorhabditis elegans Philosophical Transactions of the Royal Society B. ,vol. 314, pp. 1- 340 ,(1986) , 10.1098/RSTB.1986.0056
Wubin Qu, Changhong Ren, Yuan Li, Jinping Shi, Jiye Zhang, Xiaolei Wang, Xingyi Hang, Yiming Lu, Dongsheng Zhao, Chenggang Zhang, None, Reliability analysis of the Ahringer Caenorhabditis elegans RNAi feeding library: a guide for genome- wide screens BMC Genomics. ,vol. 12, pp. 170- 170 ,(2011) , 10.1186/1471-2164-12-170