Development of Microsatellite Markers for the Coastal Shrub Scaevola taccada (Goodeniaceae)
作者： Haruko Ando , Naoko Emura , Tetsuro Denda , Naoyuki Nakahama , Miho Inoue-Murayama
DOI: 10.3732/APPS.1300094
关键词: Goodeniaceae 、 Pyrosequencing 、 Microsatellite 、 Locus (genetics) 、 Genetics 、 Population 、 Seed dispersal 、 Genetic structure 、 Evolutionary biology 、 Biology 、 Scaevola taccada
摘要: Premise of the study: Microsatellite markers were developed for coastal shrub species Scaevola taccada to estimate population genetic structure, which may reflect different seed dispersal patterns. Methods and Results: Thirteen microsatellite primer sets S. using 454 pyrosequencing. The tested on 64 individuals sampled from two populations in Japan. Fragments amplified primers, with one 10 alleles per locus, expected heterozygosity ranged 0.00 0.85. Conclusions: These results indicate utility broad estimations structure this species.
sci-hub.se 参考文章(8)
Steve Rozen, Helen Skaletsky, Primer3 on the WWW for general users and for biologist programmers. Methods of Molecular Biology. ,vol. 132, pp. 365- 386 ,(2000) , 10.1385/1-59259-192-2:365
J. Goudet, FSTAT (Version 1.2): A Computer Program to Calculate F-Statistics Journal of Heredity. ,vol. 86, pp. 485- 486 ,(1995) , 10.1093/OXFORDJOURNALS.JHERED.A111627
Hiroki Nakanishi, Dispersal ecology of the maritime plants in the Ryukyu Islands, Japan Ecological Research. ,vol. 3, pp. 163- 173 ,(1988) , 10.1007/BF02346937
Naoko Emura, Kazuto Kawakami, Tomohiro Deguchi, Koichi Sone, Potential role of frugivorous birds in the recovery process of forest vegetation after feral goat eradication in Mukojima Island, the Bonin Islands Journal of Forest Research. ,vol. 17, pp. 352- 359 ,(2012) , 10.1007/S10310-011-0300-7
Kazuto Kawakami, Leiko Mizusawa, Hiroyoshi Higuchi, Re-established mutualism in a seed-dispersal system consisting of native and introduced birds and plants on the Bonin Islands, Japan Ecological Research. ,vol. 24, pp. 741- 748 ,(2009) , 10.1007/S11284-008-0543-8
D. G. Howarth, M. H. G. Gustafsson, D. A. Baum, T. J. Motley, Phylogenetics of the genus Scaevola (Goodeniaceae): implication for dispersal patterns across the Pacific Basin and colonization of the Hawaiian Islands American Journal of Botany. ,vol. 90, pp. 915- 923 ,(2003) , 10.3732/AJB.90.6.915
BRANT C. FAIRCLOTH, msatcommander: detection of microsatellite repeat arrays and automated, locus-specific primer design Molecular Ecology Resources. ,vol. 8, pp. 92- 94 ,(2008) , 10.1111/J.1471-8286.2007.01884.X
Isabelle Boutin-Ganache, Marco Raposo, Martine Raymond, Christian F. Deschepper, M13-tailed primers improve the readability and usability of microsatellite analyses performed with two different allele-sizing methods. BioTechniques. ,vol. 31, pp. 24- 28 ,(2001) , 10.2144/01311BM02