Anti-IL-4 monoclonal antibody prevents antibiotics-induced active fatal anaphylaxis.

摘要: We previously reported that anti-IL-4 mAb (11B11) failed to prevent protein-induced fatal murine anaphylaxis. To investigate the effect of on hapten-induced anaphylaxis, a model anaphylaxis induced by antibiotics, penicillin V (Pen V) and cephalothin (CET), was developed, observed. Pen CET 100 70 90% reactions, respectively, when C57BL/6 mice were sensitized i.p. with 500 microg antibiotic-OVA conjugate 2 x 10(9) Bordetella pertussis 1.0 mg alum challenged i.v. antibiotic-BSA 14 days later. Serum taken from passively normal develop systemic this ability serum abrogated heating at 56 degrees C for h or depletion IgE, but not IgG, Abs. Thus, antibiotic-induced reaction an IgE-dependent anaphylactic reaction. Administration beginning sensitization completely prevented reactions both CET. This associated its suppressive activity antibiotic-specific levels. More importantly, therapy in also effective preventing rapidly reduced established IgE study provides new animal indicates blocking IL-4 may be beneficial allergic diseases caused variety haptens which Abs play major role.