An Electrochemical Sandwich Immunosensor for the Detection of HER2 using Antibody-Conjugated PbS Quantum Dot as a label.

摘要: Abstract A facile electrochemical sandwich immunosensor for the detection of a breast cancer biomarker, human epidermal growth factor receptor 2 (HER2), was designed, using lead sulfide quantum dots-conjugated secondary HER2 antibody (Ab2-PbS QDs) as label. Using Ab2-PbS QDs in development immunoassays leads to many advantages such straightforward synthesis and well-defined stripping signal Pb(II) through acid dissolution, which turn yields better sensing performance sandwiched immunosensor. In bioconjugation PbS QDs, available amine hydroxyl groups from anti-HER2 capped were bound covalently together via carbonyldiimidazole (CDI) acting linker. order quantify SWV obtained, where Pb2+ ions after dissolution HCl detected. The plated mercury film SPCE also detected situ. Under optimal conditions, linear range 1–100 ng/mL with limit 0.28 ng/mL. measures satisfactory recoveries 91.3% 104.3% spiked samples, displaying high selectivity. Therefore, this method can be applied determine serum.