Sensitive detection of pyoverdine with an electrochemical sensor based on electrochemically generated graphene functionalized with gold nanoparticles.

摘要: The design and development of an electrochemical sensor for the sensitive selective determination pyoverdine, a virulence factor secreted by Pseudomonas aeruginosa, bacteria involved in nosocomial infections is presented this work. presence pyoverdine water body fluids samples can be directly linked to bacteria, thus being nontoxic low cost marker detection pollution as well biological contamination other media. was elaborated using layer-by-layer technique deposition graphene‑gold nanoparticles composite film on graphite-based screen printed electrode, from aqueous suspension. Under optimal conditions, signal corresponding oxidation process proportional its concentration, showing wide linear range 1 100μmolL-1 limit 0.33μmolL-1. This discriminate with satisfactory recoveries target analyte different real matrices also exhibited response interfering species, proving that promising medical environmental applications. In addition, proposed nanocomposite platform good reproducibility, high long term stability, sensitivity remain unchanged after stored at 4°C four weeks.