Purinergic signaling creates an anti-inflammatory profile in spleens of grass carp Ctenopharyngodon idella naturally infected by Saprolegnia parasitica: An attempt to prevent ATP pro-inflammatory effects.

摘要: Extracellular adenosine triphosphate (ATP) is as key mediator of immune and inflammatory responses. ATP normally sequestered in the intracellular milieu released by apoptotic necrotic cells, where it acts a pro-inflammatory extracellular milieu. A limited number studies have explored involvement purinergic signaling oomycete infections, including Saprolegnia parasitica; this most destructive pathogen, associated with high mortality severe economic losses for fish producers. The aim study was to determine whether exerts anti- or effects spleens grass carp (Ctenopharyngodon idella) naturally infected S. parasitica. Animals parasitica showed typical gross lesions characterized cotton-wool tufts on tail fins, well histopathological such necrosis. Spleen metabolites nitric oxide (NOx) levels were higher compared control day 7 post-infection (PI). nucleoside diphosphohydrolase (NTPDase) activity (ATP substrate) greater than uninfected PI, while no significant differences observed between groups respect NTPDase (adenosine diphosphate 5'-nucleotidase activities. Finally, deaminase (ADA) lower PI. In summary, spleen tissue necrosis context saprolegniosis provokes an intense release into milieu, interacts P2X7 purine receptor leads self-sustained deleterious cycle, contributing process. response excessive hydrolysis modulated attempt restrict process via upregulation downregulation ADA We conclude that pathway modulates responses during natural infection