Distinguishing Xyloglucanase Activity in endo-β(1→4)glucanases

摘要: Abstract The ability of β-glucanases to cleave xyloglucans, a family highly decorated β-glucans ubiquitous in plant biomass, has traditionally been overlooked functional biochemical studies. An emerging body data indicates, however, that spectrum xyloglucan specificity resides diverse glycoside hydrolases from range carbohydrate-active enzyme families—including classic “cellulase” families. This chapter outlines series kinetic and product analysis methods establish degrees modes action glycosidases discovery projects.