Transcriptional Responses in Root and Leaf of Prunus persica under Drought Stress Using RNA Sequencing

摘要: Prunus persica L. Batch, or peach, is one of the most important crops and it widely established in irrigated arid semi-arid regions. However, due to variations climate increased aridity, drought has become a major constraint, causing crop losses worldwide. The use drought-tolerant rootstocks modern fruit production appears be useful method alleviating water deficit problems. transcriptomic variation molecular mechanisms that underlie adaptation shortage remain unclear. Hence, this study, high-throughput sequencing (RNA-seq) was performed assess changes key genes involved response root tissues (GF677 rootstock) leaf (graft, var. Catherina) subjected 16 days stress. In total, 12 RNA libraries were constructed sequenced. This generated total 315M raw reads from both tissues, which allowed assembly 22,079 17,854 associated with respectively. Subsets 500 differentially expressed (DEGs) roots 236 leaves identified functionally annotated 56 gene ontology (GO) terms 99 metabolic pathways, mostly aminobenzoate degradation phenylpropanoid biosynthesis. GO analysis highlighted biological functions exclusive tissue, such as “locomotion”, “hormone process”, “detection stimulus”, indicating stress-buffering role GF677 rootstock. Furthermore, complex regulatory network revealed, involving proteins are signaling transduction, transcription hormone regulation, redox homeostasis, frontline barriers. We two poorly characterized P. persica: growth-regulating factor 5 (GRF5), may cellular expansion, AtHB12, elongation. reliability RNA-seq experiment validated by analyzing expression patterns 34 DEGs potentially tolerance using RT-qPCR. resources study provide broad characterization acclimation drought, shedding light on responses environmental stressor.