Preparation and characterization of vanillin-crosslinked chitosan therapeutic bioactive microcarriers
作者： Qin Zou , Junfeng Li , Yubao Li
DOI: 10.1016/J.IJBIOMAC.2015.05.037
关键词: Drug delivery 、 Chemistry 、 In vivo 、 Chitosan 、 Schiff base 、 Biocompatibility 、 Vanillin 、 Microcarrier 、 Emulsion 、 Nuclear chemistry
摘要: Chitosan microspheres with diameter of 14.3-48.5 μm were prepared by emulsion method and using natural vanillin as cross-linking agent. The surface morphology microstructure the characterized scanning electron microscopy, X-ray diffraction Fourier-transform infrared spectroscopy, etc. hollow showed a well-defined spherical shape median 30.3 possessed uniform micro-wrinkles, which is in favor drug release. Interpenetrating network mechanism might result from Schiff base reaction acetalization hydroxyl carbonyl between chitosan vanillin. Berberine, model drug, was loaded released sustainable manner. loading ratio could change 9.16% to 29.70% corresponding entrapment efficiency 91.61% 74.25%. In vitro cell culture study MG63 cells vivo implantation clearly that provide favorable attachment biocompatibility. results confirm drug-loaded vanillin-crosslinked be worthy candidate either carriers drugs cells, or therapeutic matrix for bone repair regeneration.
elsevier.com
sci-hub.se 参考文章(46)
K.C. Gupta, Fawzi Habeeb Jabrail, Glutaraldehyde and glyoxal cross-linked chitosan microspheres for controlled delivery of centchroman Carbohydrate Research. ,vol. 341, pp. 744- 756 ,(2006) , 10.1016/J.CARRES.2006.02.003
Jia Ma, Yogeshwar Sahai, Chitosan biopolymer for fuel cell applications. Carbohydrate Polymers. ,vol. 92, pp. 955- 975 ,(2013) , 10.1016/J.CARBPOL.2012.10.015
J. Berger, M. Reist, J.M. Mayer, O. Felt, N.A. Peppas, R. Gurny, Structure and interactions in covalently and ionically crosslinked chitosan hydrogels for biomedical applications European Journal of Pharmaceutics and Biopharmaceutics. ,vol. 57, pp. 19- 34 ,(2004) , 10.1016/S0939-6411(03)00161-9
Sharon Ansboro, Jessica S. Hayes, Valerie Barron, Shane Browne, Linda Howard, Udo Greiser, Pierce Lalor, Fintan Shannon, Frank P. Barry, Abhay Pandit, J. Mary Murphy, A chondromimetic microsphere for in situ spatially controlled chondrogenic differentiation of human mesenchymal stem cells. Journal of Controlled Release. ,vol. 179, pp. 42- 51 ,(2014) , 10.1016/J.JCONREL.2014.01.023
Peter X. Ma, Biomimetic materials for tissue engineering. Advanced Drug Delivery Reviews. ,vol. 60, pp. 184- 198 ,(2008) , 10.1016/J.ADDR.2007.08.041
Preeti V. Kulkarni, Jathi Keshavayya, Venkatrao H. Kulkarni, Effect of method of preparation and process variables on controlled release of insoluble drug from chitosan microspheres Polymers for Advanced Technologies. ,vol. 18, pp. 814- 821 ,(2007) , 10.1002/PAT.940
Vuk Uskoković, Tejal A. Desai, Simultaneous Bactericidal and Osteogenic Effect of Nanoparticulate Calcium Phosphate Powders Loaded with Clindamycin on Osteoblasts Infected with Staphylococcus Aureus Materials Science and Engineering: C. ,vol. 37, pp. 210- 222 ,(2014) , 10.1016/J.MSEC.2014.01.008
R van Dijkhuizen-Radersma, S.C Hesseling, P.E Kaim, K de Groot, J.M Bezemer, Biocompatibility and degradation of poly(ether-ester) microspheres: in vitro and in vivo evaluation. Biomaterials. ,vol. 23, pp. 4719- 4729 ,(2002) , 10.1016/S0142-9612(02)00220-X
Riccardo A.A. Muzzarelli, Chitins and chitosans for the repair of wounded skin, nerve, cartilage and bone Carbohydrate Polymers. ,vol. 76, pp. 167- 182 ,(2009) , 10.1016/J.CARBPOL.2008.11.002
Junfeng Li, Yubao Li, Jiangang Xiao, Qin Zou, The mechanism of the release of DDP from DDP-loaded n-HA/CS composite hollow microspheres Journal of Controlled Release. ,vol. 172, ,(2013) , 10.1016/J.JCONREL.2013.08.097