Distinct binding properties of eaeA-negative verocytotoxin-producing Escherichia coli of serotype O113:H21.

摘要: Infection of humans with verotoxin-producing Escherichia coli (VTEC) O113:H21 is associated clinical features comparable to those infection attaching and effacing VTEC strains including serotype O157:H7. We have shown previously that the adhesion phenotype O157:H7 influenced by presence a homolog chromosomal eaeA (for E. effacing) gene. In contrast, colony blot hybridization, negative for Therefore, aim this study was define strain CL-15 both cultured epithelial cells (HEp-2) rabbit intestine in vivo. Under transmission electron microscopy, areas microvillus effacement were observed regions directly beneath organism CL-15-infected vitro However, F-actin pedestals on host plasma membrane absent. Failure induce polymerization actin confirmed using staining fluorescein-labeled phalloidin. indirect immunofluorescence HEp-2 also failed demonstrate recruitment another cytoskeletal element, alpha-actinin, below foci bacterial adhesion. increased alpha-actinin immunofluorescence. These findings suggest factors distinct from EaeA are necessary O113:H21.