The effect of high ionic strength on monomers, polyribosomes, and puromycin-treated polyribosomes.
作者： Ester A. Zylber , Sheldon Penman
DOI: 10.1016/0005-2787(70)90505-8
关键词: Ionic strength 、 Biophysics 、 Puromycin 、 Crystallography 、 Ribosomal RNA 、 Protein biosynthesis 、 Low ionic strength 、 Monomer 、 Polysome 、 Chemistry 、 Ribosome
摘要: Abstract 1. Monomeric ribosomes of mammalian cells are quantitatively dissociated at high ionic strength. Ribosomes active in protein synthesis stable under these conditions. 2. Inactive produced by a number techniques 3. Ribosomal subunits strength reassociate rapidly when exposed to low buffer. 4. Polyribosomes normally contain only which from briefly puromycin dissociate
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sci-hub.se 参考文章(7)
T.W. Borun, M.D. Scharff, E. Robbins, Preparation of mammalian polyribosomes with the detergent Nonidet P-40 Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis. ,vol. 149, pp. 302- 304 ,(1967) , 10.1016/0005-2787(67)90715-0
S. Penman, RNA metabolism in the HeLa cell nucleus. Journal of Molecular Biology. ,vol. 17, pp. 117- 130 ,(1966) , 10.1016/S0022-2836(66)80098-0
H. Eagle, Amino acid metabolism in mammalian cell cultures. Science. ,vol. 130, pp. 432- 437 ,(1959) , 10.1126/SCIENCE.130.3373.432
Eliora Z. Ron, Robert E. Kohler, Bernard D. Davis, Magnesium ion dependence of free and polysomal ribosomes from Escherichia coli Journal of Molecular Biology. ,vol. 36, pp. 83- 89 ,(1968) , 10.1016/0022-2836(68)90221-0
William McCormick, Sheldon Penman, Inhibition of RNA synthesis in HeLa and L cells by Mengovirus Virology. ,vol. 31, pp. 135- 141 ,(1967) , 10.1016/0042-6822(67)90017-7
William McCormick, Sheldon Penman, Regulation of protein synthesis in HeLa cells: Translation at elevated temperatures☆ Journal of Molecular Biology. ,vol. 39, pp. 315- 333 ,(1969) , 10.1016/0022-2836(69)90320-9
Production of Malignancy in Vitro. IV. The Mouse Fibroblast Cultures and Changes Seen in the Living Cells Journal of the National Cancer Institute. ,vol. 4, pp. 165- 212 ,(1943) , 10.1093/JNCI/4.2.165