Water stable metal-organic framework packed microcolumn for online sorptive extraction and direct analysis of naproxen and its metabolite from urine sample.

摘要: The metal-organic framework MIL-101 was fabricated in a polyetheretherketone (PEEK) tube as micro-trapping device, and applied to sorptive extraction of naproxen its metabolite urine samples. remarkable water stability the characterizes material being different from other moisture sensitive framework. It is therefore suitable for pharmaceuticals biological fluids. adsorption isotherms aqueous solution showed that on endothermic. Additionally, exhibited higher capacity than C18-bonded silica multi-walled nanotube. A specially designed in-tube (ITSE) device endows process with characteristic rapidness, convenience, easy conjunction high performance liquid chromatography (HPLC). Finally based ITSE method coupled HPLC fluorescence detection analysis 6-O-desmethylnaproxen Parameters influence online procedure, including pH sample solution, flow rate extraction, volume, desorption solvents time were investigated. proved be highly linear range 0.05-6.0μgL(-1) limits 0.034 0.011μgL(-1) 6-O-desmethylnaproxen, respectively. recoveries samples 85.3-98.3% 94.0-97.3% intra- inter-day RSDs 2.7-5.2% 7.1-8.1%, Urine could directly subjected without any additional pretreatment. proposed demonstrated an efficient, flexible versatile tool which ideally chromatographic methods.