Factors regulating the production of vasopressin-associated human neurophysin by small-cell carcinoma of the lung: evaluation by computer-enhanced quantitative immunocytochemistry.

摘要: Abstract Expression of the vasopressin gene appears to be a property common all small-cell lung tumours. For some cultures carcinoma (SCCL), Northern and Western Blot analyses have revealed that expression this its protein products are regulated by cAMP glucocorticoids. In study, these evaluations been extended examining production vasopressin-associated human neurophysin (VP-HNP) computer-enhanced quantitative immunocytochemistry in classical cell-line (H69) SCCL, defining amount cells area positive staining above an arbitrarily set threshold. Intracellular was raised incubating with either 8,Br-cAMP (0.5 mM) IBMX mM), or forskolin (25 μM) mM). Both treatments caused significant increase VP-HNP immunoreactivity cells, further enhanced simultaneous administration dexamethasone (0.1 μM). Addition alone, however, decrease levels. Results confirm earlier findings from analysis revealing influence agents on gene-related proteins H69 indicate can effectively used provide suitable index production.