Transcriptional Responses Associated with Virulence and Defence in the Interaction between Heterobasidion annosum s.s. and Norway Spruce.

摘要: Heterobasidion annosum sensu lato is a serious pathogen causing root and stem rot to conifers in the northern hemisphere rendering timber defective for sawing pulping. In this study we applied next-generation sequencing i) identify transcriptional responses unique Heterobasidion-inoculated Norway spruce ii) investigate H. transcripts putative virulence factors. To address these objectives wounded or inoculated 30-year-old clones with 454-sequenced transcriptome of interaction at 0, 5 15 days post inoculation. The 491860 high-quality reads were de novo assembled relative expression was analysed. Overall, very few represented our dataset. Three delta-12 fatty acid desaturase one Clavaminate synthase-like transcript, both associated other pathosystems, found among significantly induced transcripts. analysis produced handful differentially expressed Most originated from genes known respond annosum. However, three that had not previously been reported showed specific induction inoculation: an oxophytodienoic acid–reductase (OPR), beta–glucosidase germin-like protein (GLP2) gene. Even small data set like ours, five novel highly without significant alignment any annotated Genbank but present P. abies (v1.0) gene catalogue identified. Their pattern suggests role defence. Therefore more complete survey interactions between its major would probably provide better understanding gymnosperm defence than accumulated until now.