Control of Mammalian Cell and Bacteria Adhesion on Substrates Micropatterned with Poly(ethylene glycol) Hydrogels
作者： Won-Gun Koh , Alexander Revzin , Aleksandr Simonian , Tony Reeves , Michael Pishko
关键词: Resist 、 Chemical engineering 、 Methacrylate 、 Substrate (chemistry) 、 Ethylene glycol 、 Materials science 、 PEG ratio 、 Monolayer 、 Adhesion 、 Self-healing hydrogels 、 Nanotechnology
摘要: A simple method for controlling the spatial positioning of mammalian cells and bacteria on substrates using patterned poly(ethylene glycol) (PEG) hydrogel microstructures is described. These were fabricated photolithography silicon, glass or poly (dimethylsiloxane) (PDMS) surfaces modified with a 3-(trichlorosilyl) propyl methacrylate (TPM) monolayer. During photogelation reaction, resulting covalently bound to substrate via TPM monolayer did not detached from upon hydration. For cell patterning, microwell arrays different dimensions fabricated. microwells composed hydrophilic PEG walls surrounding hydrophobic floors inside microwells. Murine 3T3 fibroblasts transformed hepatocytes shown selectively adhere microwells, maintaining their viability, while adherent present walls. The number one could be controled by changing lateral dimension thus allowing only single per if desired. In case 30×30 μm as many 400 in 1 mm2. addition, also effectively resist adhesion such Escherichia coli.
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