On the epidemiology and evolution of white spot syndrome virus of shrimp

摘要: WSSV causes a devastating disease in shrimp aquaculture that has spread worldwide and probably increased virulence over time. Understanding epidemiology evolution is therefore important for developing novel intervention management strategies. Both of these goals require finding suitable molecular markers to identify discriminate strains, hereby help infer their origin track spread. Five major variable genomic loci were evaluated as virus identification on different spatiotemporal scales. In this thesis the genetic variation between isolates from key production regions Vietnam was analyzed. A statistically supported model suggests multiple introductions occurred central Vietnam, radiated out time south north. Spurious generated during cloning VNTR sequences, while no replicates PCR amplification VNTRs. Moreover, sequences stable two passages infection vivo, indicating vivo can be applied study heterogeneity within originating single shrimp. Genetic deletion region variants appear more extensive farms compare intensive time, farm practices affect evolutionary dynamics WSSV. Asian provides support genome size according geometric adaptation, where incrementally smaller deletions are substituted The relationship data first occurrence implies transportation played an role quick, long range Overall, results show effectively employed However, have properties choice marker pertinent question critical.